Rv3660c Septum site-determining protein MinD @ possible CpaE

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3660c Septum site-determining protein MinD @ possible CpaE CDS 4098096 4099148 - 1 053 350 FALSE

Rv3660c (Septum site-determining protein MinD @ possible CpaE) is predicted to be co-regulated in modules bicluster_0064 with residual 0.44 and bicluster_0124 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 500.00 and 2,500.00 for bicluster_0064 and 0.06 and 1,200.00 for bicluster_0124 respectively.

These modules are enriched for following go terms: protein secretion, secretion by cell, secretion, establishment of localization in cell, cellular localization, antibiotic metabolic process, drug metabolic process, protein transport, establishment of protein localization .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.549 311 4099480 4099169 4099148
Last update: 10/16/2017 - 16:15
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14729 MT3760 478
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
2382
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610796 NP_218177.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.680000 4.23

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: