Rv3696c Glycerol kinase (EC 2.7.1.30)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3696c glpK Glycerol kinase (EC 2.7.1.30) CDS 4138202 4139755 - 1 554 517 FALSE

Rv3696c (Glycerol kinase (EC 2.7.1.30)) is predicted to be co-regulated in modules bicluster_0341 with residual 0.37 and bicluster_0533 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 590.00 and 780.00 for bicluster_0341 and 12.00 and 60.00 for bicluster_0533 respectively.

These modules are enriched for following go terms: phosphate-containing compound metabolic ..., phosphorus metabolic process, organophosphate biosynthetic process, coenzyme biosynthetic process, cofactor biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0341
e.value: 
590
Motif Bicluster: 
e.value: 
780
Motif Bicluster: 
0.37
bicluster_0533
e.value: 
12
Motif Bicluster: 
e.value: 
60
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Glycerol kinase glycerol kinase
Operon # Operon
2409
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Glycerol kinase Glycerolipid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycerolipid metabolism

11
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610832 NP_218213.1 Run
GO:0004370

glycerol kinase activity

glycerol kinase activity

Details: 
Catalysis of the reaction: ATP + glycerol = sn-glycerol 3-phosphate + ADP + 2 H(+).
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 95.36

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: