Rv3721c DNA polymerase III subunits gamma and tau (EC 2.7.7.7)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3721c dnaZX DNA polymerase III subunits gamma and tau (EC 2.7.7.7) CDS 4164995 4166731 - 1 737 578 FALSE

Rv3721c (DNA polymerase III subunits gamma and tau (EC 2.7.7.7)) is predicted to be co-regulated in modules bicluster_0321 with residual 0.54 and bicluster_0396 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 380.00 and 15,000.00 for bicluster_0321 and 0.01 and 9.30 for bicluster_0396 respectively.

These modules are enriched for following go terms: organic hydroxy compound transport, tetracycline transport, antibiotic transport, toxin transport, drug transport, response to drug, tetracycline transporter activity, drug:hydrogen antiporter activity, tetracycline:hydrogen antiporter activit..., toxin transporter activity, antibiotic transporter activity, organic hydroxy compound transmembrane t..., solute:cation antiporter activity, solute:hydrogen antiporter activity, antiporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0321
e.value: 
380
Motif Bicluster: 
e.value: 
15000
Motif Bicluster: 
0.54
bicluster_0396
e.value: 
0.0058
Motif Bicluster: 
e.value: 
9.3
Motif Bicluster: 
0.50
Product (LegacyBRC) Product (RefSeq)
DNA polymerase III subunit gamma_tau DNA polymerase III subunits gamma and tau
Operon # Operon
2426
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

DNA-directed DNA polymerase Pyrimidine metabolism, Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

DNA replication

14
Total items in this category:  

KEGG

Mismatch repair

19
Total items in this category:  

KEGG

Homologous recombination

22
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610857 NP_218238.1 Run
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: