Rv3722c Aspartate transaminase (EC 2.6.1.1)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3722c Aspartate transaminase (EC 2.6.1.1) CDS 4166821 4168128 - 1 308 435 FALSE

Rv3722c (Aspartate transaminase (EC 2.6.1.1)) is predicted to be co-regulated in modules bicluster_0082 with residual 0.54 and bicluster_0439 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,300.00 and 10,000.00 for bicluster_0082 and 0.00 and 0.00 for bicluster_0439 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -124 -0.16 0.448646 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0082
e.value: 
1300
Motif Bicluster: 
e.value: 
10000
Motif Bicluster: 
0.54
bicluster_0439
e.value: 
0.00021
Motif Bicluster: 
e.value: 
0.0026
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
2427
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117147 NP_218239.2 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: