Rv3752c tRNA-specific adenosine-34 deaminase (EC 3.5.4.-)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3752c tRNA-specific adenosine-34 deaminase (EC 3.5.4.-) CDS 4199247 4199705 - 459 152 FALSE

Rv3752c (tRNA-specific adenosine-34 deaminase (EC 3.5.4.-)) is predicted to be co-regulated in modules bicluster_0267 with residual 0.52 and bicluster_0437 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.05 for bicluster_0267 and 700.00 and 1,900.00 for bicluster_0437 respectively.

These modules are enriched for following go terms: carboxylic acid biosynthetic process, organic acid biosynthetic process, histidine biosynthetic process, histidine metabolic process, imidazole-containing compound metabolic ..., cellular amino acid metabolic process, small molecule biosynthetic process, single-organism biosynthetic process, aromatic amino acid family biosynthetic ... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE CYTIDINE_DEOXYCYTIDYLATE DEAMINASE cytidine/deoxycytidylate deaminase
Operon # Operon
2453 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

In cyclic amidines. Atrazine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Atrazine degradation

4
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610888 NP_218269.1 Run
GO:0008270

zinc ion binding

zinc ion binding

Details: 
Interacting selectively and non-covalently with zinc (Zn) ions.
GO Category: 
molecular_function
15
Total items in this category:  
GO:0016787

hydrolase activity

hydrolase activity

Details: 
Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. Hydrolase is the systematic name for any enzyme of EC class 3.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: