Rv3754 Arogenate dehydrogenase (EC 1.3.1.43)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3754 tyrA Arogenate dehydrogenase (EC 1.3.1.43) CDS 4200421 4201326 + 906 301 FALSE

Rv3754 (Arogenate dehydrogenase (EC 1.3.1.43)) is predicted to be co-regulated in modules bicluster_0123 with residual 0.55 and bicluster_0267 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 4,200.00 for bicluster_0123 and 0.00 and 0.05 for bicluster_0267 respectively.

These modules are enriched for following go terms: small molecule biosynthetic process, single-organism biosynthetic process, fatty acid biosynthetic process, carboxylic acid metabolic process, fatty acid synthase activity carboxylic acid biosynthetic process, organic acid biosynthetic process, histidine biosynthetic process, histidine metabolic process, imidazole-containing compound metabolic ..., cellular amino acid metabolic process, small molecule biosynthetic process, single-organism biosynthetic process, aromatic amino acid family biosynthetic ....

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.853 27 4200355 4200382 4200421
Product (LegacyBRC) Product (RefSeq)
PREPHENATE DEHYDROGENASE TYRA [PDH] [HYDROXYPHENYLPYRUVATE SYNTHASE] prephenate dehydrogenase
Operon # Operon
2454
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Phenylalanine, tyrosine and tryptophan biosynthesis

18
Total items in this category:  

KEGG

Novobiocin biosynthesis

3
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610890 NP_218271.1 Run
GO:0008977

prephenate dehydrogenase activity

prephenate dehydrogenase activity

Details: 
Catalysis of the reaction: NAD(+) + prephenate = (4-hydroxyphenyl)pyruvate + CO(2) + NADH.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: