Rv3776

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3776 CDS 4221089 4222648 + 1 560 519 FALSE

Rv3776 () is predicted to be co-regulated in modules bicluster_0250 with residual 0.47 and bicluster_0281 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 91.00 and 3,000.00 for bicluster_0250 and 140.00 and 1,200.00 for bicluster_0281 respectively.

These modules are enriched for following go terms: single-organism process, nucleic acid metabolic process, hydrolase activity, endodeoxyribonuclease activity, serine-type endopeptidase activity, nucleoside-triphosphatase activity, pyrophosphatase activity, hydrolase activity, acting on acid anhyd..., hydrolase activity, acting on acid anhyd..., deoxyribonuclease activity, ATPase activity, coupled, DNA-dependent ATPase activity, DNA helicase activity, serine-type peptidase activity, serine hydrolase activity acetate-CoA ligase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 04/16/2015 - 11:30
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18254 MT3885 1862
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
2473
PATRIC Locus Tag Enzyme Name Transcriptomics
NA
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.050000 1.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: