Rv3784 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3784 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) CDS 4230256 4231236 + 981 326 FALSE

Rv3784 (dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)) is predicted to be co-regulated in modules bicluster_0041 with residual 0.83 and bicluster_0533 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 48.00 for bicluster_0041 and 12.00 and 60.00 for bicluster_0533 respectively.

These modules are enriched for following go terms: biosynthetic process, cellular amide metabolic process, monocarboxylic acid metabolic process, amide binding, amino acid binding, vitamin binding phosphate-containing compound metabolic ..., phosphorus metabolic process, organophosphate biosynthetic process, coenzyme biosynthetic process, cofactor biosynthetic process.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -66 -0.49 0.652851 CDS
Motif 1 Motif 2 Residual
bicluster_0041
e.value: 
0.00027
Motif Bicluster: 
e.value: 
48
Motif Bicluster: 
0.83
bicluster_0533
e.value: 
12
Motif Bicluster: 
e.value: 
60
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
POSSIBLE dTDP-GLUCOSE 46-DEHYDRATASE dTDP-glucose 4,6-dehydratase
Operon # Operon
2477 Rv3784
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Polyketide sugar unit biosynthesis

6
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117156 YP_178015.1 Run
GO:0008460

dTDP-glucose 4,6-dehydratase activity

dTDP-glucose 4,6-dehydratase activity

Details: 
Catalysis of the reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H(2)O.
GO Category: 
molecular_function
2
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.010000 1.04

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.