Rv3792 putative membrane protein

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3792 aftA putative membrane protein CDS 4237932 4239863 + 1 932 643 FALSE

Rv3792 (putative membrane protein) is predicted to be co-regulated in modules bicluster_0068 with residual 0.53 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 20.00 and 470.00 for bicluster_0068 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: methyltransferase activity, transferase activity, transferring one-c... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.661 4238004 4237932
Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18888 MT3899.1 3251
Product (LegacyBRC) Product (RefSeq)
Arabinofuranosyltransferase aftA transmembrane protein
Operon # Operon
2481 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610928 NP_218309.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0016757

transferase activity, transferring glycosyl groups

transferase activity, transferring glycosyl groups

Details: 
Catalysis of the transfer of a glycosyl group from one compound (donor) to another (acceptor).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0035250

UDP-galactosyltransferase activity

UDP-galactosyltransferase activity

Details: 
Catalysis of the transfer of a galactose group from UDP-galactose to an acceptor molecule.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0071766

Actinobacterium-type cell wall biogenesis

Actinobacterium-type cell wall biogenesis

Details: 
A cellular process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of a cell wall of the type found in Actinobacteria. The cell wall is the rigid or semi-rigid envelope lying outside the cell membrane. Actinobacterial cell walls contain characteristic mycolic acids, of which some are covalently linked to the cell wall peptidoglycan and others accumulate at the cell surface.
GO Category: 
biological_process
19
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: