Rv3797 Probable acyl-CoA dehydrogenase fadE35 (EC 1.3.99.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3797 fadE35 Probable acyl-CoA dehydrogenase fadE35 (EC 1.3.99.-) CDS 4251085 4252866 + 1 782 593 FALSE

Rv3797 (Probable acyl-CoA dehydrogenase fadE35 (EC 1.3.99.-)) is predicted to be co-regulated in modules bicluster_0332 with residual 0.53 and bicluster_0376 with residual 0.43.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 590.00 for bicluster_0332 and 3,100.00 and 24,000.00 for bicluster_0376 respectively.

These modules are enriched for following go terms: purine nucleotide biosynthetic process, purine nucleotide metabolic process, purine-containing compound biosynthetic ... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17582 MT3904 1777
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -112 0.14 0.999609 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0332
e.value: 
0.02
Motif Bicluster: 
e.value: 
590
Motif Bicluster: 
0.53
bicluster_0376
e.value: 
3100
Motif Bicluster: 
e.value: 
24000
Motif Bicluster: 
0.43
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACYL-CoA DEHYDROGENASE FADE35 acyl-CoA dehydrogenase FADE35
Operon # Operon
2484
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610933 NP_218314.1 Run
GO:0003995

acyl-CoA dehydrogenase activity

acyl-CoA dehydrogenase activity

Details: 
Catalysis of the reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor.
GO Category: 
molecular_function
8
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.550000 1.28

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: