Rv3852 POSSIBLE HISTONE-LIKE PROTEIN HNS

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3852 hns POSSIBLE HISTONE-LIKE PROTEIN HNS CDS 4325074 4325478 + 405 134 FALSE

Rv3852 (POSSIBLE HISTONE-LIKE PROTEIN HNS) is predicted to be co-regulated in modules bicluster_0229 with residual 0.54 and bicluster_0503 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 170.00 and 860.00 for bicluster_0229 and 2.50 and 5,400.00 for bicluster_0503 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:12
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14881 MT3967 159
Product (LegacyBRC) Product (RefSeq)
POSSIBLE HISTONE-LIKE PROTEIN HNS [Putative uncharacterized protein] histone-like protein HNS
Operon # Operon
2521 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610988 NP_218369.1 Run
GO:0003677

DNA binding

DNA binding

Details: 
Any molecular function by which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
molecular_function
37
Total items in this category:  
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1427050 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1427051 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1427052 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv3852_B644 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.770000 1.59

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.144524
p-value INH: 0.852778
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 8.60 U
D3I 3 3 3.83 9.86 I
D3U 3 3 3.83 9.68 U
D5I 9 5 6.00 12.49 I
D5U 17 5 6.00 12.70 U
D7I 18 7 8.14 6.44 I
D7U 19 7 8.14 6.80 U
D14I 4 14 15.63 6.58 I
D14U 4 14 15.63 7.95 U
D17I 3 17 19.15 5.12 I
D17U 3 17 19.15 7.29 U
D21I 4 21 23.23 5.01 I
D21U 4 21 23.23 7.71 U
D24I 3 24 26.60 3.04 I
D24U 3 24 26.60 6.21 U
D28I 4 28 30.61 3.29 I
D28U 4 28 30.61 6.81 U