Rv3854c monooxygenase, flavin-binding family

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3854c ethA monooxygenase, flavin-binding family CDS 4326004 4327473 - 1 470 489 FALSE

Rv3854c (monooxygenase, flavin-binding family) is predicted to be co-regulated in modules bicluster_0129 with residual 0.53 and bicluster_0478 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 2.40 for bicluster_0129 and 0.00 and 0.09 for bicluster_0478 respectively.

These modules are enriched for following go terms: single-organism process, transport, establishment of localization, localization, arsenite transmembrane transporter activ..., anion transmembrane transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17865 MT3969 1314
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
Repressed 17 -1.27 0.000491609 Primary.TSS
Transcriptional regulator, ArsR family
No -142 0.57 0.0206185 Primary.TSS
Transcriptional repressor EthR, TetR family
Repressed 31 -2.99 3.96e-20 Primary.TSS
Transcriptional regulator, TetR family
No 33 -0.21 0.735842 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0129
e.value: 
0.0000044
Motif Bicluster: 
e.value: 
2.4
Motif Bicluster: 
0.53
bicluster_0478
e.value: 
0.00045
Motif Bicluster: 
e.value: 
0.09
Motif Bicluster: 
0.49
Product (LegacyBRC) Product (RefSeq)
MONOOXYGENASE ETHA monooxygenase ETHA
Operon # Operon
2522
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610990 NP_218371.1 Run
GO:0004499

N,N-dimethylaniline monooxygenase activity

N,N-dimethylaniline monooxygenase activity

Details: 
Catalysis of the reaction: N,N-dimethylaniline + NADPH + H+ + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0055114

oxidation-reduction process

oxidation-reduction process

Details: 
A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.
GO Category: 
biological_process
19
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.720000 2.52

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: