Rv3859c Glutamate synthase [NADPH] large chain (EC 1.4.1.13)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3859c gltB Glutamate synthase [NADPH] large chain (EC 1.4.1.13) CDS 4331498 4336081 - 4 584 1 527 FALSE

Rv3859c (Glutamate synthase [NADPH] large chain (EC 1.4.1.13)) is predicted to be co-regulated in modules bicluster_0142 with residual 0.59 and bicluster_0515 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 420.00 and 7,300.00 for bicluster_0142 and 240.00 and 390.00 for bicluster_0515 respectively.

These modules are enriched for following go terms: DNA topological change, glutamate metabolic process, dicarboxylic acid biosynthetic process, DNA conformation change, DNA topoisomerase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE [NADPH] [LARGE SUBUNIT] GLTB [L-GLUTAMATE SYNTHASE] [L-GLUTAMATE SYNTHETASE] [NADH-GLUTAMATE SYNTHASE] [GLUTAMATE SYNTHASE [NADH]][NADPH-GOGAT] ferredoxin-dependent glutamate synthase [NADPH] large subunit
Operon # Operon
2525 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Nitrogen metabolism

24
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610995 NP_218376.1 Run
GO:0004355

glutamate synthase (NADPH) activity

glutamate synthase (NADPH) activity

Details: 
Catalysis of the reaction: 2 L-glutamate + NADP(+) = 2-oxoglutarate + L-glutamine + H(+) + NADPH. This is a two-step reaction: (a) L-glutamate + NH3 = L-glutamine + H2O, (b) L-glutamate + NADP+ + H2O = NH3 + 2-oxoglutarate + NADPH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: