Rv3873 Putative ESX-1 secretion system gating protein PPE68

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3873 PPE68 Putative ESX-1 secretion system gating protein PPE68 CDS 4351075 4352181 + 1 107 368 FALSE

Rv3873 (Putative ESX-1 secretion system gating protein PPE68) is predicted to be co-regulated in modules bicluster_0243 with residual 0.50 and bicluster_0559 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.06 and 2,000.00 for bicluster_0243 and 0.00 and 0.00 for bicluster_0559 respectively.

These modules are enriched for following go terms: Mo-molybdopterin cofactor biosynthetic p..., Mo-molybdopterin cofactor metabolic proc..., molybdopterin cofactor biosynthetic proc..., molybdopterin cofactor metabolic process, prosthetic group metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.008 4351066 4351075
Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14748 MT3987 68
Product (LegacyBRC) Product (RefSeq)
PPE FAMILY PROTEIN PPE family protein
Operon # Operon
2532 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117164 YP_178022.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.920000 1.81

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: