Rv3910 Proposed peptidoglycan lipid II flippase MurJ

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3910 Proposed peptidoglycan lipid II flippase MurJ CDS 4396597 4400151 + 3 555 1 184 FALSE

Rv3910 (Proposed peptidoglycan lipid II flippase MurJ) is predicted to be co-regulated in modules bicluster_0131 with residual 0.45 and bicluster_0472 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 58.00 and 110.00 for bicluster_0131 and 0.01 and 1.70 for bicluster_0472 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.364 3498 4400095 425 4396193 4396618 4396597
Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14907 MT4029 221
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Mycofactocin system transcriptional regulator
No 0 0.03 0.999588 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0131
e.value: 
58
Motif Bicluster: 
e.value: 
110
Motif Bicluster: 
0.45
bicluster_0472
e.value: 
0.0071
Motif Bicluster: 
e.value: 
1.7
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED TRANSMEMBRANE PROTEIN transmembrane protein
Operon # Operon
2551 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Peptidoglycan biosynthesis

16
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15611046 NP_218427.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.180000 2.12

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: