Rv3913 Thioredoxin reductase (EC 1.8.1.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3913 trxB2 Thioredoxin reductase (EC 1.8.1.9) CDS 4401728 4402735 + 1 008 335 FALSE

Rv3913 (Thioredoxin reductase (EC 1.8.1.9)) is predicted to be co-regulated in modules bicluster_0056 with residual 0.52 and bicluster_0546 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.11 and 16,000.00 for bicluster_0056 and 0.00 and 0.21 for bicluster_0546 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on a sul..., helicase activity, oxidoreductase activity, acting on a sul... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Thioredoxin reductase thioredoxin reductase TRXB2
Operon # Operon
2552 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Thioredoxin-disulfide reductase Selenoamino acid metabolism, Pyrimidine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyrimidine metabolism

41
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15611049 NP_218430.1 Run
GO:0004791

thioredoxin-disulfide reductase activity

thioredoxin-disulfide reductase activity

Details: 
Catalysis of the reaction: NADP(+) + thioredoxin = H(+) + NADPH + thioredoxin disulfide.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0001666

response to hypoxia

response to hypoxia

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
biological_process
37
Total items in this category:  
GO:0004791

thioredoxin-disulfide reductase activity

thioredoxin-disulfide reductase activity

Details: 
Catalysis of the reaction: NADP(+) + thioredoxin = H(+) + NADPH + thioredoxin disulfide.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0045454

cell redox homeostasis

cell redox homeostasis

Details: 
Any process that maintains the redox environment of a cell or compartment within a cell.
GO Category: 
biological_process
9
Total items in this category:  
GO:0050660

flavin adenine dinucleotide binding

flavin adenine dinucleotide binding

Details: 
Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0070402

NADPH binding

NADPH binding

Details: 
Interacting selectively and non-covalently with the reduced form, NADPH, of nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: