Rv3915 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3915 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) CDS 4403192 4404412 + 1 221 406 FALSE

Rv3915 (N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)) is predicted to be co-regulated in modules bicluster_0081 with residual 0.55 and bicluster_0520 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.53 and 19,000.00 for bicluster_0081 and 330.00 and 7,500.00 for bicluster_0520 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE HYDROLASE hydrolase
Operon # Operon
2553
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117169 YP_178027.1 Run
GO:0008745

N-acetylmuramoyl-L-alanine amidase activity

N-acetylmuramoyl-L-alanine amidase activity

Details: 
Catalysis of the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain bacterial cell-wall glycopeptides.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0033922

peptidoglycan beta-N-acetylmuramidase activity

peptidoglycan beta-N-acetylmuramidase activity

Details: 
Catalysis of the hydrolysis of terminal, non-reducing N-acetylmuramic residues.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: