Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0493 hupV

hydrogenase large subunit (NCBI)

CircVis
Functional Annotations (4)
Function System
Ni,Fe-hydrogenase I large subunit cog/ cog
electron transport go/ biological_process
ferredoxin hydrogenase activity go/ molecular_function
nickel ion binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0493
(Mouseover regulator name to see its description)

RSP_0493 is regulated by 33 influences and regulates 0 modules.
Regulators for RSP_0493 hupV (33)
Regulator Module Operator
RSP_0014 213 tf
RSP_0402 213 tf
RSP_0415 213 tf
RSP_0457 213 tf
RSP_0591 213 tf
RSP_0616 213 tf
RSP_1163 213 tf
RSP_1231 213 tf
RSP_1590 213 tf
RSP_1704 213 tf
RSP_1945 213 tf
RSP_2780 213 tf
RSP_3022 213 tf
RSP_3024 213 tf
RSP_3109 213 tf
RSP_3124 213 tf
RSP_3448 213 tf
RSP_3528 213 tf
RSP_3731 213 tf
RSP_0068 265 tf
RSP_0186 265 tf
RSP_0402 265 tf
RSP_1077 265 tf
RSP_1225 265 tf
RSP_1231 265 tf
RSP_1866 265 tf
RSP_1945 265 tf
RSP_2351 265 tf
RSP_2889 265 tf
RSP_2922 265 tf
RSP_2950 265 tf
RSP_3448 265 tf
RSP_3684 265 tf

Warning: RSP_0493 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8146 1.30e+02 tttTG.aTatctt
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8147 1.20e+03 ACggA.AaCccGatCGAaa
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8248 6.10e+04 ATaaTT
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8249 8.90e+03 CcgATCaT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0493

RSP_0493 is enriched for 4 functions in 3 categories.
Enrichment Table (4)
Function System
Ni,Fe-hydrogenase I large subunit cog/ cog
electron transport go/ biological_process
ferredoxin hydrogenase activity go/ molecular_function
nickel ion binding go/ molecular_function
Module neighborhood information for RSP_0493

RSP_0493 has total of 34 gene neighbors in modules 213, 265
Gene neighbors (34)
Gene Common Name Description Module membership
RSP_0205 RSP_0205 hypothetical protein (NCBI) 213, 327
RSP_0216 RSP_0216 hypothetical protein (NCBI) 189, 213
RSP_0307 RSP_0307 Antifreeze protein, type I (NCBI) 133, 265
RSP_0350 RSP_0350 hypothetical protein (NCBI) 140, 213
RSP_0493 hupV hydrogenase large subunit (NCBI) 213, 265
RSP_0592 RSP_0592 Putative lipase/esterase (NCBI) 99, 213
RSP_0594 RSP_0594 Putative choline sulfatase (NCBI) 265, 293
RSP_0602 RSP_0602 Possible adenosyl cobinamide kinase/ cobinamide phosphate guanylyltransferase (NCBI) 213, 350
RSP_0603 RSP_0603 hypothetical protein (NCBI) 213, 350
RSP_0867 rnhA ribonuclease H (NCBI) 213, 327
RSP_0903 RSP_0903 Catalase (NCBI) 69, 265
RSP_0947 RSP_0947 hypothetical protein (NCBI) 168, 213
RSP_1503 RSP_1503 hypothetical protein (NCBI) 265, 267
RSP_1525 RSP_1525 putative Helicase/Exonuclease (NCBI) 42, 265
RSP_1673 RSP_1673 Probable holo-acyl carrier protein synthase (NCBI) 265, 267
RSP_1850 ptsI phosphoenolpyruvate-protein phosphotransferase, PtsI(Ntr) (NCBI) 213, 240
RSP_1950 RSP_1950 hypothetical protein (NCBI) 213, 383
RSP_2038 RSP_2038 possible acetyltransferase (NCBI) 213, 372
RSP_2075 RSP_2075 Putative Transcriptional regulator (NCBI) 54, 213
RSP_2213 RSP_2213 hypothetical protein (NCBI) 159, 265
RSP_2240 RSP_2240 hypothetical protein (NCBI) 213, 340
RSP_2281 RSP_2281 two-component trancriptional regulator, winged helix family (NCBI) 94, 265
RSP_2362 RSP_2362 transcriptional regulator, GntR family (NCBI) 174, 265
RSP_2373 RSP_2373 Putative Transporter, Major facilitator superfamily (MFS) (NCBI) 135, 265
RSP_2377 tdh L-threonine 3-dehydrogenase (NCBI) 152, 265
RSP_2870 aglK ABC alpha-glucoside transporter, ATPase subunit AglK (NCBI) 120, 265
RSP_2915 phoR Probable two-component sensor histidine kinase (NCBI) 213, 214
RSP_2957 RSP_2957 hypothetical protein (NCBI) 168, 213
RSP_3022 RSP_3022 transcriptional regulator, TetR family (NCBI) 213, 323
RSP_3082 RSP_3082 hypothetical protein (NCBI) 185, 213
RSP_3088 RSP_3088 two component transcriptional regulator, winged helix family (NCBI) 176, 213
RSP_3301 eda 2-dehydro-3-deoxyphosphogluconate aldolase (NCBI) 265, 267
RSP_3771 RSP_3771 hypothetical protein (NCBI) 72, 213
RSP_3809 RSP_3809 hypothetical protein (NCBI) 177, 213
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0493
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend