Organism : Bacillus subtilis | Module List :
BSU15690 rpoZ

DNA-directed RNA polymerase subunit omega (RefSeq)

CircVis
Functional Annotations (14)
Function System
DNA-directed RNA polymerase, subunit K/omega cog/ cog
DNA binding go/ molecular_function
DNA-directed RNA polymerase activity go/ molecular_function
DNA-directed RNA polymerase I activity go/ molecular_function
DNA-directed RNA polymerase II activity go/ molecular_function
DNA-directed RNA polymerase III activity go/ molecular_function
transcription, DNA-dependent go/ biological_process
regulation of transcription, DNA-dependent go/ biological_process
RNA polymerase complex go/ cellular_component
Purine metabolism kegg/ kegg pathway
Pyrimidine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
RNA polymerase kegg/ kegg pathway
rpoZ tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BSU15690
(Mouseover regulator name to see its description)

BSU15690 is regulated by 15 influences and regulates 17 modules.
Regulators for BSU15690 rpoZ (15)
Regulator Module Operator
BSU00800 272 tf
BSU15690 272 tf
BSU16170 272 tf
BSU16600 272 tf
BSU24250 272 tf
BSU29740 272 tf
BSU00800 288 tf
BSU15690 288 tf
BSU16170 288 tf
BSU16600 288 tf
BSU16810 288 tf
BSU24250 288 tf
BSU37290 288 tf
BSU37620 288 tf
BSU40670 288 tf
Regulated by BSU15690 (17)
Module Residual Genes
44 0.48 30
63 0.43 28
71 0.46 26
88 0.30 14
97 0.48 25
106 0.34 19
111 0.43 24
114 0.47 24
155 0.52 21
158 0.38 22
160 0.38 24
172 0.35 17
175 0.36 22
178 0.42 24
225 0.41 24
272 0.37 21
288 0.36 19
Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
5482 9.90e+00 GCtCgGCAtGCaA
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5483 5.40e+03 CGGACG
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5514 5.30e+02 gctccgCa.gcaAaA
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5515 1.30e+03 gG.AA.A.CGtg
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BSU15690

BSU15690 is enriched for 14 functions in 3 categories.
Enrichment Table (14)
Function System
DNA-directed RNA polymerase, subunit K/omega cog/ cog
DNA binding go/ molecular_function
DNA-directed RNA polymerase activity go/ molecular_function
DNA-directed RNA polymerase I activity go/ molecular_function
DNA-directed RNA polymerase II activity go/ molecular_function
DNA-directed RNA polymerase III activity go/ molecular_function
transcription, DNA-dependent go/ biological_process
regulation of transcription, DNA-dependent go/ biological_process
RNA polymerase complex go/ cellular_component
Purine metabolism kegg/ kegg pathway
Pyrimidine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
RNA polymerase kegg/ kegg pathway
rpoZ tigr/ tigrfam
Module neighborhood information for BSU15690

BSU15690 has total of 28 gene neighbors in modules 272, 288
Gene neighbors (28)
Gene Common Name Description Module membership
BSU00340 yabB putative methyltransferase (RefSeq) 225, 288
BSU00350 yazA GIY-YIG nuclease superfamily protein (RefSeq) 225, 288
BSU00360 yabC putative methyltransferase (RefSeq) 225, 288
BSU15660 yloC hypothetical protein (RefSeq) 225, 288
BSU15670 ylzA hypothetical protein (RefSeq) 272, 288
BSU15680 gmk guanylate kinase (RefSeq) 272, 288
BSU15690 rpoZ DNA-directed RNA polymerase subunit omega (RefSeq) 272, 288
BSU15700 coaBC Coenzyme A biosynthesis bifunctional protein CoaBC; phosphopantothenoylcysteine synthetase/decarboxylase (RefSeq) 272, 288
BSU15710 priA primosome assembly protein PriA (RefSeq) 272, 288
BSU15720 def peptide deformylase (RefSeq) 272, 288
BSU15730 fmt methionyl-tRNA formyltransferase (RefSeq) 272, 288
BSU15740 rsmB RNA-binding Sun protein; 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (RefSeq) 272, 288
BSU15750 yloN ribosomal RNA large subunit methyltransferase N (RefSeq) 272, 288
BSU16150 clpQ ATP-dependent protease peptidase subunit (RefSeq) 272, 273
BSU16160 hslU ATP-dependent protease ATP-binding subunit HslU (RefSeq) 272, 273
BSU16760 dapG aspartate kinase I (RefSeq) 106, 272
BSU16770 dapA dihydrodipicolinate synthase (RefSeq) 106, 272
BSU19380 yojO putative activator of nitric oxide reductase (RefSeq) 272, 288
BSU23610 nudF ADP-ribose pyrophosphatase (RefSeq) 272, 292
BSU24240 recN factor for double strand breaks DNA repair and genetic recombination (RefSeq) 272, 288
BSU24250 ahrC arginine repressor (RefSeq) 272, 288
BSU24260 yqxC putative methyltransferase with RNA binding domain (RefSeq) 211, 288
BSU24270 dxs 1-deoxy-D-xylulose-5-phosphate synthase (RefSeq) 211, 288
BSU27710 tgt queuine tRNA-ribosyltransferase (RefSeq) 221, 272
BSU27720 queA S-adenosylmethionine:tRNA ribosyltransferase-isomerase (RefSeq) 221, 272
BSU30800 menB naphthoate synthase (RefSeq) 272, 273
BSU30830 menF menaquinone-specific isochorismate synthase (RefSeq) 212, 272
VIMSS36718 VIMSS36718 None 288, 289
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BSU15690
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend