Organism : Clostridium acetobutylicum | Module List :
Predicted transcriptional regulator (NCBI ptt file)
Functional Annotations (1)
|sequence-specific DNA binding||go/ molecular_function|
Regulation information for CAC1559(Mouseover regulator name to see its description)
Motif information (de novo identified motifs for modules)
There are 4 motifs predicted.
|Motif Id||e-value||Consensus||Motif Logo|
Functional Enrichment for CAC1559
|sequence-specific DNA binding||go/ molecular_function|
Module neighborhood information for CAC1559
|Gene||Common Name||Description||Module membership|
|CAC0089||serA||D-3 phosphoglycerate dehydrogenase (NCBI ptt file)||109, 343|
|CAC0090||CAC0090||Xylanase/chitin deacetylase, NodB family (NCBI ptt file)||109, 312|
|CAC0225||CAC0225||Sensory transduction histidine kinase (NCBI ptt file)||57, 109|
|CAC0277||CAC0277||HD-GYP hydrolase domain containing protein (NCBI ptt file)||109, 298|
|CAC0278||CAC0278||Aspartate kinase (NCBI ptt file)||85, 151|
|CAC0415||CAC0415||TPR-repeat-containing protein (NCBI ptt file)||151, 217|
|CAC0416||CAC0416||TPR-repeat-containing protein (NCBI ptt file)||151, 217|
|CAC0452||CAC0452||Permease (NCBI ptt file)||99, 109|
|CAC0506||CAC0506||Penicillin-binding protein (NCBI ptt file)||151, 215|
|CAC0507||CAC0507||Predicted metal-dependent membrane hydrolase (NCBI ptt file)||151, 215|
|CAC0585||CAC0585||N-terminal CheY reciever domain fused to C-terminal uncharacterized CheX-like domain (NCBI ptt file)||66, 151|
|CAC0633||CAC0633||Predicted phosphatase (NCBI ptt file)||82, 151|
|CAC0641||CAC0641||Uncharacterized conserved protein (NCBI ptt file)||109, 348|
|CAC0685||CAC0685||Putative Mn transporter, NRAMP family (NCBI ptt file)||151, 215|
|CAC0826||CAC0826||Endoglucanase family 5 (NCBI ptt file)||151, 359|
|CAC0849||CAC0849||Predicted transcriptional regulator (NCBI ptt file)||151, 216|
|CAC0961||CAC0961||Cobyric acid synthase CobQ (NCBI ptt file)||151, 201|
|CAC0962||CAC0962||UDP-N-acetylmuramyl tripeptide synthetase (NCBI ptt file)||79, 151|
|CAC1098||polA||DNA polymerase I, polA (NCBI ptt file)||99, 109|
|CAC1172||CAC1172||Predicted integrase of XerC/XerD family, diverged (NCBI ptt file)||151, 238|
|CAC1297||CAC1297||N-terminal fragment of elongation factor Ts (NCBI ptt file)||49, 109|
|CAC1312||CAC1312||Hypothetical protein (NCBI ptt file)||109, 176|
|CAC1318||CAC1318||Hypothetical protein (NCBI ptt file)||151, 252|
|CAC1404||CAC1404||Transcriptional regulator of sugar metabolism (deoR family) (NCBI ptt file)||64, 109|
|CAC1538||CAC1538||Predicted permease, YCXC B.subtilis ortholog (NCBI ptt file)||59, 151|
|CAC1539||CAC1539||Gamma-glutamylcysteine synthetase (NCBI ptt file)||109, 222|
|CAC1559||CAC1559||Predicted transcriptional regulator (NCBI ptt file)||109, 151|
|CAC1635||CAC1635||Predicted nucleic acid binding protein, containing 2 S1 domains, YITL B.subtilis ortholog (NCBI ptt file)||109, 312|
|CAC1651||CAC1651||Predicted GTPase with uncharacterized domain, ortholog of T.maritima (4980952) (NCBI ptt file)||109, 283|
|CAC1652||aspA||Aspartate ammonia-lyase (NCBI ptt file)||109, 329|
|CAC1661||CAC1661||Predicted secreted nucleic acid binding protein (NCBI ptt file)||25, 109|
|CAC1741||CAC1741||Uncharacterized conserved protein, YLBM B.subtilis ortholog (NCBI ptt file)||151, 270|
|CAC1955||CAC1955||Hypothetical protein (NCBI ptt file)||57, 109|
|CAC1965||greA||Transcription elongation factor GreA (NCBI ptt file)||63, 109|
|CAC2037||CAC2037||Hypothetical protein, CF-39 family (NCBI ptt file)||109, 312|
|CAC2437||CAC2437||Uncharacterized conserved protein (NCBI ptt file)||100, 109|
|CAC2787||CAC2787||Serine/threonine protein phosphatase (NCBI ptt file)||109, 128|
|CAC2873||CAC2873||Acetyl-CoA acetyltransferase (NCBI ptt file)||10, 109|
|CAC3152||CAC3152||DNA-dependent RNA polymerase sigma subunit (NCBI ptt file)||5, 109|
|CAC3155||CAC3155||Uncharacterized conserved protein, THY1 family (NCBI ptt file)||19, 109|
|CAC3221||prs||Phosphoribosylpyrophosphate synthetase (NCBI ptt file)||109, 251|
|CAC3247||CAC3247||Predicted transcriptional regulator, lacI/xre family (NCBI ptt file)||151, 359|
|CAC3263||CAC3263||Hypothetical protein (NCBI ptt file)||52, 151|
|CAC3307||CAC3307||TPR-repeats containing protein (NCBI ptt file)||109, 190|
|CAC3352||CAC3352||Membrane associated methyl-accepting chemotaxis protein with HAMP domain (NCBI ptt file)||151, 217|
|CAC3602||CAC3602||HD superfamily hydrolase (NCBI ptt file)||86, 109|
|CAC3675||CAC3675||Hypothetical secreted protein (NCBI ptt file)||106, 151|
Gene Page Help
If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.
Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
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Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.
If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.
You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".
For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.
Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.
Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.
Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.
Module Members Tab
Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.
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CircVisOur circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
- 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
- 2. Source gene
- 3. Target genes (other module members)
- 4. Interactions between source and target genes for a particular module
- 5. Module(s) that source gene and target genes belong to
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