Organism : Geobacter sulfurreducens | Module List :
GSU0520

conserved hypothetical protein (VIMSS)

CircVis
Functional Annotations (6)
Function System
Uncharacterized conserved protein cog/ cog
RNA binding go/ molecular_function
pseudouridylate synthase activity go/ molecular_function
pseudouridine synthase activity go/ molecular_function
tRNA pseudouridine synthesis go/ biological_process
tRNA_TruD_broad tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU0520
(Mouseover regulator name to see its description)

GSU0520 is regulated by 25 influences and regulates 0 modules.
Regulators for GSU0520 (25)
Regulator Module Operator
GSU0013 212 tf
GSU0581 212 tf
GSU1013 212 tf
GSU1522 212 tf
GSU1525 212 tf
GSU1626 212 tf
GSU1687 212 tf
GSU1934 212 tf
GSU2185 212 tf
GSU2520 212 tf
GSU2523 212 tf
GSU3363 212 tf
GSU0018 40 tf
GSU0063 40 tf
GSU0205 40 tf
GSU0581 40 tf
GSU1129 40 tf
GSU1410 40 tf
GSU1586 40 tf
GSU1831 40 tf
GSU2625 40 tf
GSU2753 40 tf
GSU3060 40 tf
GSU3298 40 tf
GSU3396 40 tf

Warning: GSU0520 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2240 2.80e+02 TgCCG.c.a.cGc
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2241 5.70e+03 CcGtaGTTCacTTtT
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2584 1.60e+04 ATAAAAAGACCGTA
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2585 1.70e+04 GggcTTaTcTTGTct
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU0520

GSU0520 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Uncharacterized conserved protein cog/ cog
RNA binding go/ molecular_function
pseudouridylate synthase activity go/ molecular_function
pseudouridine synthase activity go/ molecular_function
tRNA pseudouridine synthesis go/ biological_process
tRNA_TruD_broad tigr/ tigrfam
Module neighborhood information for GSU0520

GSU0520 has total of 43 gene neighbors in modules 40, 212
Gene neighbors (43)
Gene Common Name Description Module membership
GSU0002 recF recF protein (NCBI) 212, 301
GSU0083 GSU0083 conserved hypothetical protein TIGR00726 (VIMSS) 3, 40
GSU0224 GSU0224 conserved hypothetical protein (VIMSS) 40, 258
GSU0460 fabF-1 3-oxoacyl-(acyl-carrier-protein) synthase II (NCBI) 40, 114
GSU0474 GSU0474 sensory box/GGDEF family protein (VIMSS) 40, 253
GSU0513 GSU0513 dephospho-CoA kinase (VIMSS) 212, 219
GSU0520 GSU0520 conserved hypothetical protein (VIMSS) 40, 212
GSU0879 cheV chemotaxis protein CheV (NCBI) 8, 212
GSU0997 mutM formamidopyrimidine-DNA glycosylase (NCBI) 40, 164
GSU1111 GSU1111 conserved hypothetical protein TIGR00048 (VIMSS) 139, 212
GSU1116 GSU1116 conserved domain protein (VIMSS) 212, 267
GSU1195 GSU1195 HD domain protein (VIMSS) 199, 212
GSU1197 GSU1197 RNA methyltransferase, TrmH family, group 2, putative (NCBI) 40, 47
GSU1208 GSU1208 membrane protein, putative (NCBI) 67, 212
GSU1249 GSU1249 sensory box histidine kinase (VIMSS) 40, 113
GSU1269 GSU1269 hypothetical protein (VIMSS) 40, 159
GSU1273 carA carbamoyl-phosphate synthase, small subunit (NCBI) 39, 212
GSU1301 cheW-5 purine-binding chemotaxis protein CheW (NCBI) 212, 299
GSU1358 GSU1358 conserved hypothetical protein (VIMSS) 199, 212
GSU1361 GSU1361 Piwi domain protein (NCBI) 212, 299
GSU1421 GSU1421 nuclease SbcCD, D subunit, putative (NCBI) 212, 219
GSU1422 GSU1422 nuclease SbcCD, C subunit, putative (NCBI) 212, 219
GSU1548 GSU1548 hypothetical protein (VIMSS) 40, 253
GSU1898 GSU1898 membrane protein, putative (VIMSS) 29, 212
GSU1901 GSU1901 hypothetical protein (VIMSS) 29, 212
GSU1935 birA birA biofunctional protein, putative (NCBI) 98, 212
GSU2192 cbbZ phosphoglycolate phosphatase (NCBI) 4, 40
GSU2200 GSU2200 hypothetical protein (VIMSS) 40, 289
GSU2341 GSU2341 NADH-ubiquinone/plastoquinone family protein (VIMSS) 40, 289
GSU2367 GSU2367 organic solvent tolerance protein, putative (NCBI) 98, 212
GSU2431 GSU2431 membrane protein, putative (NCBI) 40, 49
GSU2434 lipB lipoate-protein ligase B (NCBI) 40, 128
GSU2451 GSU2451 PilB-related protein (VIMSS) 40, 258
GSU2452 GSU2452 copper-translocating P-type ATPase (VIMSS) 40, 59
GSU2475 GSU2475 sigma-54 dependent transcriptional regulator (NCBI) 40, 49
GSU2517 GSU2517 rhodanese-like domain/cysteine-rich domain protein (NCBI) 43, 212
GSU2520 GSU2520 (R)-2-hydroxyglutaryl-CoA dehydratase activator (VIMSS) 83, 212
GSU2575 GSU2575 sensor histidine kinase/response regulator (VIMSS) 40, 141
GSU2718 hoxH NAD-reducing hydrogenase, beta subunit (NCBI) 40, 289
GSU2721 hoxF NAD-reducing hydrogenase, alpha subunit (NCBI) 40, 128
GSU3137 GSU3137 cytochrome c family protein (NCBI) 40, 128
GSU3204 GSU3204 conserved hypothetical protein (VIMSS) 212, 299
GSU3446 GSU3446 thioredoxin family protein, selenocysteine-containing (NCBI) 40, 49
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU0520
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend