Organism : Methanococcus maripaludis S2 | Module List :
MMP0986 thyA

thymidylate synthase

CircVis
Functional Annotations (9)
Function System
Uncharacterized protein conserved in archaea cog/ cog
thymidylate synthase activity go/ molecular_function
electron transport go/ biological_process
electron carrier activity go/ molecular_function
thymidylate synthase biosynthetic process go/ biological_process
iron-sulfur cluster binding go/ molecular_function
Pyrimidine metabolism kegg/ kegg pathway
One carbon pool by folate kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for MMP0986
(Mouseover regulator name to see its description)

MMP0986 is regulated by 2 influences and regulates 0 modules.
Regulators for MMP0986 thyA (2)
Regulator Module Operator
MMP0032
MMP0907
104 combiner
MMP0097
MMP1467
104 combiner

Warning: MMP0986 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
865 3.00e+02 tgtAAc.gttccTGg
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866 3.10e+03 cCCCCG
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935 9.60e+02 cCgCcgtgAaC
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936 7.30e+03 CcAccaTTGtaaGc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for MMP0986

MMP0986 is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Uncharacterized protein conserved in archaea cog/ cog
thymidylate synthase activity go/ molecular_function
electron transport go/ biological_process
electron carrier activity go/ molecular_function
thymidylate synthase biosynthetic process go/ biological_process
iron-sulfur cluster binding go/ molecular_function
Pyrimidine metabolism kegg/ kegg pathway
One carbon pool by folate kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for MMP0986

MMP0986 has total of 51 gene neighbors in modules 104, 142
Gene neighbors (51)
Gene Common Name Description Module membership
Antisense_1 None 85, 104
MMP0008 DP1 DNA polymerase II small subunit 22, 75, 142
MMP0011 DNA-cytosine methyltransferase 4, 104
MMP0017 hypothetical protein MMP0017 67, 142
MMP0019 hypothetical protein MMP0019 14, 104, 142
MMP0021 hypothetical protein MMP0021 14, 142
MMP0077 radical SAM domain-containing protein 35, 104
MMP0113 hypothetical protein MMP0113 23, 75, 142
MMP0119 birA biotin--acetyl-CoA-carboxylase ligase 1, 75, 142
MMP0120 hypothetical protein MMP0120 55, 142
MMP0125 flpA fibrillarin-like protein 14, 104
MMP0126 bioB biotin synthase 14, 104
MMP0161 comB 2-phosphosulfolactate phosphatase 85, 104
MMP0170 cofF RimK family alpha-L-glutamate ligase 104, 133
MMP0186 hypothetical protein MMP0186 104, 133
MMP0214 putative deoxyribonucleotide triphosphate pyrophosphatase 12, 104
MMP0218 hypothetical protein MMP0218 15, 52, 104
MMP0225 gldA glycerol dehydrogenase 117, 142
MMP0236 hypothetical protein MMP0236 75, 104
MMP0324 hypothetical protein MMP0324 62, 142
MMP0379 hypothetical protein MMP0379 104, 163
MMP0380 dnaB DNA polymerase family B protein 15, 104
MMP0392 purD phosphoribosylamine--glycine ligase 4, 75, 104
MMP0544 MoaA/nifB/pqqE family protein 55, 142
MMP0545 putative molybdopterin biosynthesis protein MoeA/LysR substrate binding-domain-containing protein 55, 142
MMP0727 uvrB excinuclease ABC subunit B 49, 142
MMP0728 uvrC excinuclease ABC subunit C 90, 142
MMP0731 exodeoxyribonuclease VII small subunit 85, 104
MMP0895 hypothetical protein MMP0895 14, 104
MMP0909 hypothetical protein MMP0909 75, 104
MMP0986 thyA thymidylate synthase 104, 142
MMP1009 pyrC dihydroorotase 22, 142
MMP1027 hypothetical protein MMP1027 104, 163
MMP1062 hypothetical protein MMP1062 142, 144
MMP1080 group 1 glycosyl transferase 14, 142
MMP1141 ATP-dependent helicase 75, 142
MMP1202 hypothetical protein MMP1202 104, 153
MMP1203 cbiD cobalt-precorrin-6A synthase 104, 153
MMP1228 hypothetical protein MMP1228 4, 22, 142
MMP1232 PP-loop domain-containing protein 95, 104
MMP1236 hypothetical protein MMP1236 22, 55, 117, 142, 152
MMP1264 hypothetical protein MMP1264 104, 142, 157
MMP1346 basic helix-loop-helix dimerization domain-containing protein 49, 67, 142
MMP1350 radical SAM domain-containing protein 14, 47, 104
MMP1377 hypothetical protein MMP1377 69, 104
MMP1398 dapE diaminopimelate aminotransferase 22, 142
MMP1485 moaB molybdenum cofactor biosynthesis protein 104, 142
MMP1494 hypothetical protein MMP1494 104, 153
MMP1597 phosphatidylglycerophosphatase A 22, 142, 144
MMP1600 ribosomal protein S6 modification protein 104, 129
Unanno_59 None 28, 104, 133
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for MMP0986
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend