Organism : Clostridium acetobutylicum | Module List :
CAC3391

Membrane associated signal transduction histidine kinase (NCBI ptt file)

CircVis
Functional Annotations (6)
Function System
Signal transduction histidine kinase regulating citrate/malate metabolism cog/ cog
two-component sensor activity go/ molecular_function
ATP binding go/ molecular_function
signal transduction go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC3391
(Mouseover regulator name to see its description)

CAC3391 is regulated by 21 influences and regulates 0 modules.
Regulators for CAC3391 (21)
Regulator Module Operator
CAC0265 351 tf
CAC1766 351 tf
CAC1843 351 tf
CAC2074 351 tf
CAC2259 351 tf
CAC2793 351 tf
CAC2939 351 tf
CAC3267 351 tf
CAC3406 351 tf
CAC3413 351 tf
CAC0113 51 tf
CAC0859 51 tf
CAC1226 51 tf
CAC1264 51 tf
CAC1786 51 tf
CAC2060 51 tf
CAC2074 51 tf
CAC2084 51 tf
CAC2209 51 tf
CAC2608 51 tf
CAC3611 51 tf

Warning: CAC3391 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6756 5.80e-03 taaGGagG
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6757 2.00e+03 GGACCTGG
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7354 5.40e+00 gGaGGa
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7355 1.00e+04 CCGcAAGaG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC3391

CAC3391 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Signal transduction histidine kinase regulating citrate/malate metabolism cog/ cog
two-component sensor activity go/ molecular_function
ATP binding go/ molecular_function
signal transduction go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Module neighborhood information for CAC3391

CAC3391 has total of 53 gene neighbors in modules 51, 351
Gene neighbors (53)
Gene Common Name Description Module membership
CAC0349 CAC0349 Predicted membrane protein (NCBI ptt file) 246, 351
CAC0419 CAC0419 Predicted membrane protein (NCBI ptt file) 247, 351
CAC0698 CAC0698 Predicted hydrolase of the HAD superfamily (NCBI ptt file) 217, 351
CAC1060 CAC1060 Predicted membrane protein (NCBI ptt file) 336, 351
CAC1064 CAC1064 TPR-repeat-containing protein (NCBI ptt file) 49, 351
CAC1163 CAC1163 Hypothetical protein (NCBI ptt file) 16, 351
CAC1167 CAC1167 Hypothetical protein (NCBI ptt file) 246, 351
CAC1213 CAC1213 Predicted membrane protein (NCBI ptt file) 246, 351
CAC1292 CAC1292 Membrane protein containing HD superfamily hydrolase domain, YQFF ortholog (NCBI ptt file) 51, 283
CAC1293 CAC1293 Uncharacterized conserved predicted metal-binding protein, YQFG ortholog (NCBI ptt file) 51, 229
CAC1294 dgkA/pgpB Diacylglycerol kinase (dgkA) fused to phosphatase B domain (pgpB) (NCBI ptt file) 51, 229
CAC1305 CAC1305 Uncharacterized protein similar to a protein from Cylindrotheca fusiformis plasmid (GI:99322) (NCBI ptt file) 51, 315
CAC1306 CAC1306 Hypothetical protein (NCBI ptt file) 5, 51
CAC1307 CAC1307 Hypothetical protein (NCBI ptt file) 51, 297
CAC1308 CAC1308 Uncharacterized low-complexity protein, related to YISX/YYBG B.subtilis (NCBI ptt file) 51, 317
CAC1309 CAC1309 Hypothetical protein (NCBI ptt file) 3, 51
CAC1409 CAC1409 Predicted membrane protein (NCBI ptt file) 78, 351
CAC1500 CAC1500 Predicted membrane protein (NCBI ptt file) 13, 351
CAC1517 CAC1517 Membrane associated sensory transduction histidine kinase (NCBI ptt file) 140, 351
CAC1625 CAC1625 Phosphoserine phosphatase family protein (NCBI ptt file) 29, 51
CAC1736 recG RecG helicase (NCBI ptt file) 3, 51
CAC1794 CAC1794 Predicted permease (NCBI ptt file) 3, 51
CAC1818 CAC1818 Conserved membrane protein, possible 4-hydroxybenzoate octaprenyltranferase (NCBI ptt file) 3, 51
CAC1823 CAC1823 Uncharacterized conserved protein (NCBI ptt file) 51, 315
CAC1824 clpA ATP-dependent Clp proteinase (NCBI ptt file) 51, 315
CAC1848 cmk Cytidylate kinase (NCBI ptt file) 35, 351
CAC1972 CAC1972 Predicted membrane protein (NCBI ptt file) 191, 351
CAC1982 CAC1982 Predicted membrane protein (NCBI ptt file) 51, 317
CAC2065 deoB Phosphopentomutase (NCBI ptt file) 51, 91
CAC2066 CAC2066 Integrase/recombinase XerD family (NCBI ptt file) 51, 91
CAC2067 CAC2067 Hypothetical protein (NCBI ptt file) 51, 268
CAC2068 CAC2068 Sporulation factor spoIIM, uncharacterized membrane protein (NCBI ptt file) 51, 268
CAC2115 lspA Lipoprotein signal peptidase (NCBI ptt file) 51, 352
CAC2138 CAC2138 Exopolyphosphatase (NCBI ptt file) 2, 51
CAC2188 CAC2188 Protein containing a domain related to multimeric flavodoxin WrbA family (NCBI ptt file) 351, 355
CAC2234 nifS NifS family enzyme (cysteine desulfurase/cysteine sulfinate desulfinase) (NCBI ptt file) 51, 329
CAC2259 CAC2259 Predicted transcriptional regulator of MarR-family (NCBI ptt file) 270, 351
CAC2269 aspS Aspartyl-tRNA synthetase (NCBI ptt file) 51, 329
CAC2322 CAC2322 Hypothetical protein (NCBI ptt file) 4, 351
CAC2340 mutS1 DNA mismatch repair protein mutS, YSHD B.subtilis ortholog (NCBI ptt file) 51, 91
CAC2341 CAC2341 Collagenase family protease (NCBI ptt file) 51, 351
CAC2559 CAC2559 Hypothetical protein (NCBI ptt file) 183, 351
CAC2566 CAC2566 Possible rRNA methylase (SAM-dependent methyltransferase superfamily), YTQB B.subtilis (NCBI ptt file) 58, 351
CAC2698 CAC2698 Hypothetical protein (NCBI ptt file) 5, 351
CAC2724 CAC2724 Hypothetical protein (NCBI ptt file) 51, 278
CAC2725 CAC2725 Predicted hydrolase from alpha/beta family, YQKD B.subtilis ortholog (NCBI ptt file) 51, 225
CAC2770 CAC2770 Amino acid transporter (NCBI ptt file) 3, 51
CAC2900 splB Spore photoproduct lyase, SPL related protein (NCBI ptt file) 132, 351
CAC2901 CAC2901 Predicted membrane protein (NCBI ptt file) 298, 351
CAC2939 CAC2939 Response regulator (CheY-like receiver domain and HTH-type DNA-binding domain) (NCBI ptt file) 258, 351
CAC3390 CAC3390 Response regulator (CheY-like receiver domain containing) (NCBI ptt file) 51, 297
CAC3391 CAC3391 Membrane associated signal transduction histidine kinase (NCBI ptt file) 51, 351
CAC3404 CAC3404 Uncharactecterized conserved membrane protein, possible ABC-type MDR permease (NCBI ptt file) 246, 351
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC3391
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend