Rv0035 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0035 fadD34 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) CDS 37259 38947 + 1 689 562 FALSE

Rv0035 (Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)) is predicted to be co-regulated in modules bicluster_0410 with residual 0.47 and bicluster_0548 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.20 and 4.10 for bicluster_0410 and 0.00 and 0.26 for bicluster_0548 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE FATTY-ACID-CoA LIGASE FADD34 [FATTY-ACID-CoA SYNTHETASE] [FATTY-ACID-CoA SYNTHASE] fatty-acid-CoA ligase
Operon # Operon
20 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Long-chain-fatty-acid--CoA ligase Fatty acid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116683 YP_177686.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.100000 1.26

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: