Rv0114

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0114 gmhB CDS 137941 138513 + 573 190 FALSE

Rv0114 () is predicted to be co-regulated in modules bicluster_0380 with residual 0.52 and bicluster_0507 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.07 and 0.10 for bicluster_0380 and 0.00 and 0.00 for bicluster_0507 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
DD-heptose 17-bisphosphate phosphatase D-alpha,beta-D-heptose-1,7-biphosphate phosphatase
Operon # Operon
76 - -
PATRIC Locus Tag Enzyme Name Transcriptomics

PATRIC

NA
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Lipopolysaccharide biosynthesis

3
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
GO:0005529

NA

NA

Details: 
NA
GO Category: 
NA
2
Total items in this category:  
GO:0005975

carbohydrate metabolic process

carbohydrate metabolic process

Details: 
The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. Includes the formation of carbohydrate derivatives by the addition of a carbohydrate residue to another molecule.
GO Category: 
biological_process
4
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.830000 1.54

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: