Rv0145

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0145 CDS 171215 172168 + 954 317 FALSE

Rv0145 () is predicted to be co-regulated in modules bicluster_0331 with residual 0.41 and bicluster_0485 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0331 and 0.00 and 0.07 for bicluster_0485 respectively.

These modules are enriched for following go terms: primary metabolic process, cellular metabolic process, cellular process, organic substance metabolic process, cell, cell part, large ribosomal subunit, small ribosomal subunit .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Organic hydroperoxide resistance transcriptional regulator
No -59 -0.3 0.0995112 Primary.TSS
DNA-binding response regulator TrcR
No -43 0.15 0.629374 Primary.TSS
transcriptional regulator, ArsR family
No -14 0.3 0.00103423 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0331
e.value: 
0.0000000081
Motif Bicluster: 
e.value: 
0.00082
Motif Bicluster: 
0.41
bicluster_0485
e.value: 
0.00012
Motif Bicluster: 
e.value: 
0.068
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0145_MT0153
Operon # Operon
104
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607287 NP_214659.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.090000 0.59

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: