Rv0169 MCE-family protein Mce1A

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0169 mce1A MCE-family protein Mce1A CDS 198534 199898 + 1 365 454 FALSE

Rv0169 (MCE-family protein Mce1A) is predicted to be co-regulated in modules bicluster_0392 with residual 0.64 and bicluster_0541 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,800.00 and 3,000.00 for bicluster_0392 and 0.02 and 0.19 for bicluster_0541 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
MCE-FAMILY PROTEIN MCE1A MCE-family protein MCE1A
Operon # Operon
122 - - - - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116701 YP_177701.1 Run
GO:0003779

actin binding

actin binding

Details: 
Interacting selectively and non-covalently with monomeric or multimeric forms of actin, including actin filaments.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0007157

heterophilic cell-cell adhesion

heterophilic cell-cell adhesion

Details: 
The attachment of an adhesion molecule in one cell to a nonidentical adhesion molecule in an adjacent cell.
GO Category: 
biological_process
2
Total items in this category:  
GO:0008017

microtubule binding

microtubule binding

Details: 
Interacting selectively and non-covalently with microtubules, filaments composed of tubulin monomers.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0030260

entry into host cell

entry into host cell

Details: 
The invasion by an organism of a cell of its host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
1
Total items in this category:  
GO:0042785

active evasion of host immune response via regulation of host cytokine network

active evasion of host immune response via regulation of host cytokine network

Details: 
Any mechanism of active immune avoidance which works by regulating host cytokine networks, e.g. by secreting proteins that mimic cytokine receptors that act to sequester host cytokines and inhibit action. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
2
Total items in this category:  
GO:0044117

growth of symbiont in host

growth of symbiont in host

Details: 
The increase in size or mass of an organism, occurring within the cells or tissues of the host organism. This may (but not necessarily) include a filamentous growth form, and also can include secretion of proteases and lipases to break down host tissue. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
36
Total items in this category:  
GO:0044406

adhesion to host

adhesion to host

Details: 
The attachment of an organism to its host via adhesion molecules, general stickiness etc., either directly or indirectly. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
1
Total items in this category:  
GO:0044409

entry into host

entry into host

Details: 
Penetration by an organism into the body, tissues, or cells of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.480000 2.30

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: