Rv0173 MCE-family lipoprotein LprK (MCE-family lipoprotein Mce1e)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0173 lprK MCE-family lipoprotein LprK (MCE-family lipoprotein Mce1e) CDS 204065 205237 + 1 173 390 FALSE

Rv0173 (MCE-family lipoprotein LprK (MCE-family lipoprotein Mce1e)) is predicted to be co-regulated in modules bicluster_0162 with residual 0.54 and bicluster_0541 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 45.00 and 5,400.00 for bicluster_0162 and 0.02 and 0.19 for bicluster_0541 respectively.

These modules are enriched for following go terms: cysteine biosynthetic process from serin..., cysteine biosynthetic process, cysteine metabolic process, L-serine metabolic process .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.168 1159 205224 204068 204065
Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15459 MT0182 1806
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, IclR family
No -50 0.38 0.545137 Internal.TSS
Transcriptional regulatory protein
Repressed 42 -1.91 1.02e-16 Internal.TSS
Transcriptional regulator, TetR family
No 61 -0.48 0.292867 CDS
Motif 1 Motif 2 Residual
bicluster_0162
e.value: 
45
Motif Bicluster: 
e.value: 
5400
Motif Bicluster: 
0.54
bicluster_0541
e.value: 
0.021
Motif Bicluster: 
e.value: 
0.19
Motif Bicluster: 
0.44
Product (LegacyBRC) Product (RefSeq)
POSSIBLE MCE-FAMILY LIPOPROTEIN LPRK [MCE-FAMILY LIPOPROTEIN MCE1E] MCE-family lipoprotein LprK
Operon # Operon
122 - - - - - - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607314 NP_214687.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0044117

growth of symbiont in host

growth of symbiont in host

Details: 
The increase in size or mass of an organism, occurring within the cells or tissues of the host organism. This may (but not necessarily) include a filamentous growth form, and also can include secretion of proteases and lipases to break down host tissue. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
36
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.430000 3.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: