Rv0212c Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1) / Ribosylnicotinamide kinase (EC 2.7.1.22)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0212c nadR Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1) / Ribosylnicotinamide kinase... CDS 253669 254640 - 972 323 TRUE

Rv0212c (Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1) / Ribosylnicotinamide kinase (EC 2.7.1.22)) is predicted to be co-regulated in modules bicluster_0007 with residual 0.55 and bicluster_0021 with residual 0.46.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0007 and 0.00 and 0.00 for bicluster_0021 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 3 of 3
Gene Target Differential Expression Distance Expression pvalue Type
Transcriptional regulatory protein
No -116 0.43 0.0782481 Primary.TSS
3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41)
No -114 0.12 0.923791 Primary.TSS
ESX-1 secretion system protein (Rv3616c)
No -67 0.41 0.51669 Primary.TSS
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No 68 0.55 0.25479 Primary.TSS
Product (LegacyBRC) Product (RefSeq)
POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN NADR [PROBABLY ASNC-FAMILY] transcriptional regulatory protein NadR
Operon # Operon
147 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607353 NP_214726.1 Run
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426429 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426430 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426431 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0212c_B400 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.620000 2.07

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.781715
p-value INH: 0.863689
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 8.76 U
D3I 3 3 3.83 7.88 I
D3U 3 3 3.83 9.02 U
D5I 9 5 6.00 10.34 I
D5U 17 5 6.00 10.16 U
D7I 18 7 8.14 8.69 I
D7U 19 7 8.14 9.07 U
D14I 4 14 15.63 10.34 I
D14U 4 14 15.63 10.60 U
D17I 3 17 19.15 9.88 I
D17U 3 17 19.15 10.85 U
D21I 4 21 23.23 9.48 I
D21U 4 21 23.23 10.63 U
D24I 3 24 26.60 8.68 I
D24U 3 24 26.60 10.56 U
D28I 4 28 30.61 8.57 I
D28U 4 28 30.61 10.22 U