Rv2245 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2245 kasA 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41) CDS 2518115 2519365 + 1 251 416 FALSE

Rv2245 (3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41)) is predicted to be co-regulated in modules bicluster_0231 with residual 0.47 and bicluster_0369 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.10 and 6,500.00 for bicluster_0231 and 5.10 and 230.00 for bicluster_0369 respectively.

These modules are enriched for following go terms: lipid biosynthetic process, lipid metabolic process, transferase activity, methyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
3-oxoacyl-[acyl-carrier-protein] synthase 1 3-oxoacyl-(acyl carrier protein) synthase II
Operon # Operon
1474 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Beta-ketoacyl-acyl-carrier-protein synthase I Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609382 NP_216761.1 Run
GO:0004315

3-oxoacyl-[acyl-carrier-protein] synthase activity

3-oxoacyl-[acyl-carrier-protein] synthase activity

Details: 
Catalysis of the reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein].
GO Category: 
molecular_function
3
Total items in this category:  
GO:0004315

3-oxoacyl-[acyl-carrier-protein] synthase activity

3-oxoacyl-[acyl-carrier-protein] synthase activity

Details: 
Catalysis of the reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein].
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009923

fatty acid elongase complex

fatty acid elongase complex

Details: 
A tetrameric complex of four different subunits which catalyzes the elongation of fatty acids chains 2 carbon units at a time in the synthesis of very long chain fatty acids.
GO Category: 
cellular_component
1
Total items in this category:  
GO:0019367

fatty acid elongation, saturated fatty acid

fatty acid elongation, saturated fatty acid

Details: 
Elongation of a saturated fatty acid chain.
GO Category: 
biological_process
1
Total items in this category:  
GO:0030497

fatty acid elongation

fatty acid elongation

Details: 
The elongation of a fatty acid chain by the sequential addition of two-carbon units.
GO Category: 
biological_process
4
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: