Rv0247c Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0247c Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) CDS 298116 298862 - 747 248 FALSE

Rv0247c (Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1)) is predicted to be co-regulated in modules bicluster_0255 with residual 0.52 and bicluster_0269 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.80 for bicluster_0255 and 150.00 and 1,600.00 for bicluster_0269 respectively.

These modules are enriched for following go terms: tryptophan metabolic process, indolalkylamine metabolic process, indole-containing compound metabolic pro..., cellular biogenic amine metabolic proces..., active transmembrane transporter activit..., transporter activity.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Organic hydroperoxide resistance transcriptional regulator
No -72 -0.07 0.89195 Internal.TSS
POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN
No 64 -0.12 0.823683 Internal.TSS
No 55 0.29 0.293244 CDS
Motif 1 Motif 2 Residual
bicluster_0255
e.value: 
0.000011
Motif Bicluster: 
e.value: 
3.8
Motif Bicluster: 
0.52
bicluster_0269
e.value: 
150
Motif Bicluster: 
e.value: 
1600
Motif Bicluster: 
0.48
Product (LegacyBRC) Product (RefSeq)
PROBABLE SUCCINATE DEHYDROGENASE [IRON-SULFUR SUBUNIT] [SUCCINIC DEHYDROGENASE] fumarate reductase iron-sulfur subunit
Operon # Operon
173 Rv0247c - Rv0248c - Rv0249c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Toluene degradation

13
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607388 NP_214761.1 Run
GO:0000104

succinate dehydrogenase activity

succinate dehydrogenase activity

Details: 
Catalysis of the reaction: succinate + acceptor = fumarate + reduced acceptor.
GO Category: 
molecular_function
7
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.420000 3.00

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.