Rv0248c Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0248c sdhA Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) CDS 298863 300803 - 1 941 646 FALSE

Rv0248c (Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1)) is predicted to be co-regulated in modules bicluster_0255 with residual 0.52 and bicluster_0501 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.80 for bicluster_0255 and 14.00 and 11,000.00 for bicluster_0501 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15721 MT0262 2989
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, PadR family
No -141 -0.02 0.960834 Primary.TSS
Transcriptional regulator, TetR family
No 3 -0.03 0.957578 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0255
e.value: 
0.000011
Motif Bicluster: 
e.value: 
3.8
Motif Bicluster: 
0.52
bicluster_0501
e.value: 
14
Motif Bicluster: 
e.value: 
11000
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
PROBABLE SUCCINATE DEHYDROGENASE [IRON-SULFUR SUBUNIT] [SUCCINIC DEHYDROGENASE] succinate dehydrogenase flavoprotein subunit
Operon # Operon
173 Rv0247c - Rv0248c - Rv0249c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Toluene degradation

13
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607389 NP_214762.1 Run
GO:0000104

succinate dehydrogenase activity

succinate dehydrogenase activity

Details: 
Catalysis of the reaction: succinate + acceptor = fumarate + reduced acceptor.
GO Category: 
molecular_function
7
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.000000 4.37

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.