Rv0282 AAA family ATPase, ESX-1 secretion system component Rv3868

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0282 eccA3 AAA family ATPase, ESX-1 secretion system component Rv3868 CDS 342130 344025 + 1 896 631 FALSE

Rv0282 (AAA family ATPase, ESX-1 secretion system component Rv3868) is predicted to be co-regulated in modules bicluster_0295 with residual 0.48 and bicluster_0513 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 5,200.00 for bicluster_0295 and 130.00 and 270.00 for bicluster_0513 respectively.

These modules are enriched for following go terms: organonitrogen compound biosynthetic pro..., organonitrogen compound metabolic proces..., NAD biosynthetic process, nicotinamide nucleotide biosynthetic pro..., NAD metabolic process, pyridine nucleotide biosynthetic process, acetolactate synthase activity, transferase activity, transferring aldeh... glutamate metabolic process, dicarboxylic acid biosynthetic process, intracellular protein transport, protein secretion, secretion by cell, secretion, intracellular transport, cellular protein localization, cellular macromolecule localization, protein transmembrane transporter activi..., P-P-bond-hydrolysis-driven protein trans..., macromolecule transmembrane transporter ..., protein transporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.24 1557 343687 187 341976 342163 342130
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv0282_MT0295
Operon # Operon
196 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607423 NP_214796.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0010106

cellular response to iron ion starvation

cellular response to iron ion starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of iron ions.
GO Category: 
biological_process
14
Total items in this category:  
GO:0033214

iron assimilation by chelation and transport

iron assimilation by chelation and transport

Details: 
A process in which iron (Fe3+) is solubilized by ferric iron-specific chelators, known as siderophores, excreted by a cell; the iron-siderophore complex is then transported into the cell by specific cell surface receptors.
GO Category: 
biological_process
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: