Rv0334 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0334 rmlA Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) CDS 398658 399524 + 867 288 FALSE

Rv0334 (Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24)) is predicted to be co-regulated in modules bicluster_0078 with residual 0.48 and bicluster_0421 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 360.00 and 14,000.00 for bicluster_0078 and 360.00 and 130.00 for bicluster_0421 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0078
e.value: 
360
Motif Bicluster: 
e.value: 
14000
Motif Bicluster: 
0.48
bicluster_0421
e.value: 
360
Motif Bicluster: 
e.value: 
130
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Glucose-1-phosphate thymidylyltransferase alpha-D-glucose-1-phosphate thymidylyltransferase RmlA
Operon # Operon
230 Rv0332 - Rv0333 - Rv0334
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Streptomycin biosynthesis

12
Total items in this category:  

KEGG

Polyketide sugar unit biosynthesis

6
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607475 NP_214848.1 Run
GO:0008879

glucose-1-phosphate thymidylyltransferase activity

glucose-1-phosphate thymidylyltransferase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate + dTTP = diphosphate + dTDP-glucose.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008879

glucose-1-phosphate thymidylyltransferase activity

glucose-1-phosphate thymidylyltransferase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate + dTTP = diphosphate + dTDP-glucose.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0019300

rhamnose biosynthetic process

rhamnose biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of rhamnose, the hexose 6-deoxy-L-mannose.
GO Category: 
biological_process
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.