Rv0375c Carbon monoxide dehydrogenase medium chain (EC 1.2.99.2)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0375c Carbon monoxide dehydrogenase medium chain (EC 1.2.99.2) CDS 452294 453154 - 861 286 FALSE

Rv0375c (Carbon monoxide dehydrogenase medium chain (EC 1.2.99.2)) is predicted to be co-regulated in modules bicluster_0207 with residual 0.51 and bicluster_0283 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 150.00 and 95.00 for bicluster_0207 and 0.01 and 1.50 for bicluster_0283 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE CARBON MONOXYDE DEHYDROGENASE [MEDIUM CHAIN] carbon monoxyde dehydrogenase medium subunit
Operon # Operon
253 - - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nitrotoluene degradation

4
Total items in this category:  

KEGG

Methane metabolism

26
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607516 NP_214889.1 Run
GO:0018492

carbon-monoxide dehydrogenase (acceptor) activity

carbon-monoxide dehydrogenase (acceptor) activity

Details: 
Catalysis of the reaction: CO + H2O + acceptor = CO2 + reduced acceptor.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.670000 1.49

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: