Rv0392c NADH dehydrogenase (EC 1.6.99.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0392c ndhA NADH dehydrogenase (EC 1.6.99.3) CDS 471227 472639 - 1 413 470 FALSE

Rv0392c (NADH dehydrogenase (EC 1.6.99.3)) is predicted to be co-regulated in modules bicluster_0028 with residual 0.52 and bicluster_0357 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.50 for bicluster_0028 and 94.00 and 1,300.00 for bicluster_0357 respectively.

These modules are enriched for following go terms: signal transduction, signaling, single organism signaling, cell communication, response to stimulus, signal transducer activity, molecular transducer activity, phosphorelay sensor kinase activity, receptor activity, signaling receptor activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/15/2017 - 23:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13507 MT0403 160
Product (LegacyBRC) Product (RefSeq)
PROBABLE MEMBRANE NADH DEHYDROGENASE NDHA membrane NADH dehydrogenase
Operon # Operon
267
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

NADH dehydrogenase Oxidative phosphorylation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Oxidative phosphorylation

47
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607533 NP_214906.1 Run
GO:0003954

NADH dehydrogenase activity

NADH dehydrogenase activity

Details: 
Catalysis of the reaction: NADH + H+ + acceptor = NAD+ + reduced acceptor.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0003955

NAD(P)H dehydrogenase (quinone) activity

NAD(P)H dehydrogenase (quinone) activity

Details: 
Catalysis of the reaction: NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.940000 1.68

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: