Rv0412c Possible membrane protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0412c Possible membrane protein CDS 498300 499619 - 1 320 439 FALSE

Rv0412c (Possible membrane protein) is predicted to be co-regulated in modules bicluster_0369 with residual 0.51 and bicluster_0482 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 5.10 and 230.00 for bicluster_0369 and 0.02 and 0.93 for bicluster_0482 respectively.

These modules are enriched for following go terms: lipid biosynthetic process, lipid metabolic process, transferase activity, methyltransferase activity cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, macromolecule metabolic process, cytoplasm.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 02:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17909 MT0425 540
Product (LegacyBRC) Product (RefSeq)
POSSIBLE CONSERVED MEMBRANE PROTEIN
Operon # Operon
278 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607553 NP_214926.1 Run
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: