Rv0414c Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0414c thiE Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) CDS 500350 501018 - 669 222 FALSE

Rv0414c (Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)) is predicted to be co-regulated in modules bicluster_0113 with residual 0.50 and bicluster_0498 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.20 and 170.00 for bicluster_0113 and 0.00 and 6.30 for bicluster_0498 respectively.

These modules are enriched for following go terms: exodeoxyribonuclease activity, exodeoxyribonuclease activity, producing..., exonuclease activity, active with either..., deoxyribonuclease activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Probable thiamine-phosphate pyrophosphorylase thiamine-phosphate pyrophosphorylase
Operon # Operon
280
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Thiamine-phosphate diphosphorylase Thiamine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Thiamine metabolism

9
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607555 NP_214928.1 Run
GO:0004789

thiamine-phosphate diphosphorylase activity

thiamine-phosphate diphosphorylase activity

Details: 
Catalysis of the reaction: 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphoethyl)-thiazole + H(+) = diphosphate + thiamine phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: