Rv0416 Sulfur carrier protein ThiS

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0416 thiS Sulfur carrier protein ThiS CDS 502167 502373 + 207 68 FALSE

Rv0416 (Sulfur carrier protein ThiS) is predicted to be co-regulated in modules bicluster_0498 with residual 0.51 and bicluster_0584 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 6.30 for bicluster_0498 and 0.30 and 380.00 for bicluster_0584 respectively.

These modules are enriched for following go terms: nucleobase-containing compound biosynthe..., small molecule metabolic process, cellular nitrogen compound biosynthetic ....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE PROTEIN THIS sulfur carrier protein ThiS
Operon # Operon
281 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Sulfur relay system

18
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607557 NP_214930.1 Run
GO:0006790

sulfur compound metabolic process

sulfur compound metabolic process

Details: 
The chemical reactions and pathways involving the nonmetallic element sulfur or compounds that contain sulfur, such as the amino acids methionine and cysteine or the tripeptide glutathione.
GO Category: 
biological_process
3
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: