Rv0417 Thiazole biosynthesis protein ThiG

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0417 thiG Thiazole biosynthesis protein ThiG CDS 502366 503124 + 759 252 FALSE

Rv0417 (Thiazole biosynthesis protein ThiG) is predicted to be co-regulated in modules bicluster_0498 with residual 0.51 and bicluster_0584 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 6.30 for bicluster_0498 and 0.30 and 380.00 for bicluster_0584 respectively.

These modules are enriched for following go terms: nucleobase-containing compound biosynthe..., small molecule metabolic process, cellular nitrogen compound biosynthetic ....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Thiazole biosynthesis protein thiG thiazole synthase
Operon # Operon
281 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Thiamine metabolism

9
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607558 NP_214931.1 Run
GO:0009228

thiamine biosynthetic process

thiamine biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of thiamine (vitamin B1), a water soluble vitamin present in fresh vegetables and meats, especially liver.
GO Category: 
biological_process
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: