Rv0458 Aldehyde dehydrogenase (EC 1.2.1.3)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0458 Aldehyde dehydrogenase (EC 1.2.1.3) CDS 549675 551198 + 1 524 507 FALSE

Rv0458 (Aldehyde dehydrogenase (EC 1.2.1.3)) is predicted to be co-regulated in modules bicluster_0072 with residual 0.54 and bicluster_0438 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.32 for bicluster_0072 and 2.50 and 3,200.00 for bicluster_0438 respectively.

These modules are enriched for following go terms: porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet..., cofactor biosynthetic process, sulfur amino acid biosynthetic process, sulfur amino acid metabolic process, cofactor metabolic process.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, ArsR family
Repressed 32 -2.73 6.02e-34 Primary.TSS
Histone protein Lsr2
No 11 -0.18 0.804657 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0072
e.value: 
0.0000000033
Motif Bicluster: 
e.value: 
0.32
Motif Bicluster: 
0.54
bicluster_0438
e.value: 
2.5
Motif Bicluster: 
e.value: 
3200
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Probable aldehyde dehydrogenase aldehyde dehydrogenase
Operon # Operon
308 Rv0458 - Rv0459
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose and glucuronate interconversions

8
Total items in this category:  

KEGG

Ascorbate and aldarate metabolism

5
Total items in this category:  

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607599 NP_214972.1 Run
GO:0004029

aldehyde dehydrogenase (NAD) activity

aldehyde dehydrogenase (NAD) activity

Details: 
Catalysis of the reaction: an aldehyde + NAD+ + H2O = an acid + NADH + H+.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.110000 3.97

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.