Rv0819 Acetyl-CoA:Cys-GlcN-Ins acetyltransferase, mycothiol synthase MshD

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0819 mshD Acetyl-CoA:Cys-GlcN-Ins acetyltransferase, mycothiol synthase MshD CDS 911736 912683 + 948 315 FALSE

Rv0819 (Acetyl-CoA:Cys-GlcN-Ins acetyltransferase, mycothiol synthase MshD) is predicted to be co-regulated in modules bicluster_0226 with residual 0.52 and bicluster_0558 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 2,000.00 for bicluster_0226 and 0.00 and 0.69 for bicluster_0558 respectively.

These modules are enriched for following go terms: response to stress, S-methyltransferase activity, O-acyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
541 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607959 NP_215334.1 Run
GO:0008080

N-acetyltransferase activity

N-acetyltransferase activity

Details: 
Catalysis of the transfer of an acetyl group to a nitrogen atom on the acceptor molecule.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0006950

response to stress

response to stress

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a disturbance in organismal or cellular homeostasis, usually, but not necessarily, exogenous (e.g. temperature, humidity, ionizing radiation).
GO Category: 
biological_process
9
Total items in this category:  
GO:0010125

mycothiol biosynthetic process

mycothiol biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of mycothiol, which consists of N-acetyl-L-cysteine linked to a pseudodisaccharide, D-glucosamine and myo-inositol. Mycothiol is produced in actinomycetes like mycobacteria and serves similar functions to glutathione.
GO Category: 
biological_process
4
Total items in this category:  
GO:0035447

mycothiol synthase activity

mycothiol synthase activity

Details: 
Catalysis of the reaction: 1-D-myo-inosityl-2-L-cysteinylamido-2-deoxy-alpha-D-glucopyranoside + acetyl-CoA = mycothiol + coenzyme A + H+. Mycothiol is also known as AcCys-GlcN-Ins and 1-D-myo-inosityl-2-L-cysteinylamido-2-deoxy-alpha-D-glucopyranoside as Cys-GlcN-Ins or desacetylmycothiol.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.150000 76.35

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: