Rv0824c Fatty acid desaturase occurring in virulence cluster

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0824c desA1 Fatty acid desaturase occurring in virulence cluster CDS 917734 918750 - 1 017 338 FALSE

Rv0824c (Fatty acid desaturase occurring in virulence cluster) is predicted to be co-regulated in modules bicluster_0276 with residual 0.47 and bicluster_0426 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 93.00 and 170.00 for bicluster_0276 and 950.00 and 11,000.00 for bicluster_0426 respectively.

These modules are enriched for following go terms: drug transmembrane transporter activity, drug transporter activity nicotinamide nucleotide metabolic proces..., oxidoreduction coenzyme metabolic proces..., pyridine nucleotide metabolic process, pyridine-containing compound metabolic p..., RNA binding.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
\Transcriptional regulator, TetR family\\\""
Repressed 6 -1.76 0.000000434 Primary.TSS
Possible transcriptional regulatory protein
No -2 -0.43 0.207005 Primary.TSS
DNA-binding response regulator TrcR
No -3 0.04 0.980217 Primary.TSS
Transcriptional regulator, MarR family
No -43 0.31 0.452262 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0276
e.value: 
93
Motif Bicluster: 
e.value: 
170
Motif Bicluster: 
0.47
bicluster_0426
e.value: 
950
Motif Bicluster: 
e.value: 
11000
Motif Bicluster: 
0.50
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACYL-[ACYL-CARRIER PROTEIN] DESATURASE DESA1 [ACYL-[ACP] DESATURASE] [STEAROYL-ACP DESATURASE] [PROTEIN DES] acyl-[acyl-carrier protein] desaturase
Operon # Operon
546 Rv0824c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Biosynthesis of unsaturated fatty acids

10
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116786 YP_177758.1 Run
GO:0045300

acyl-[acyl-carrier-protein] desaturase activity

acyl-[acyl-carrier-protein] desaturase activity

Details: 
Catalysis of the reaction: stearoyl-[acyl-carrier protein] + reduced acceptor + O2 = oleoyl-[acyl-carrier protein] + acceptor + H2O. The enzyme requires ferredoxin.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.