Rv0934 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0934 pstS1 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) CDS 1042115 1043239 + 1 125 374 FALSE

Rv0934 (Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)) is predicted to be co-regulated in modules bicluster_0269 with residual 0.48 and bicluster_0480 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 150.00 and 1,600.00 for bicluster_0269 and 0.30 and 53.00 for bicluster_0480 respectively.

These modules are enriched for following go terms: tryptophan metabolic process, indolalkylamine metabolic process, indole-containing compound metabolic pro..., cellular biogenic amine metabolic proces..., active transmembrane transporter activit..., transporter activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 08:59
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14951 MT0961 346
Product (LegacyBRC) Product (RefSeq)
Phosphate-binding protein pstS 1 periplasmic phosphate-binding lipoprotein PSTS1 (PBP-1) (PSTS1)
Operon # Operon
622 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

ABC transporters

57
Total items in this category:  

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116801 YP_177770.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006817

phosphate ion transport

phosphate ion transport

Details: 
The directed movement of phosphate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
4
Total items in this category:  
GO:0016036

cellular response to phosphate starvation

cellular response to phosphate starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of phosphate.
GO Category: 
biological_process
2
Total items in this category:  
GO:0042301

phosphate ion binding

phosphate ion binding

Details: 
Interacting selectively and non-covalently with phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.93

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: