Rv1082 Mycothiol S-conjugate amidase Mca

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1082 mca Mycothiol S-conjugate amidase Mca CDS 1206520 1207386 + 867 288 FALSE

Rv1082 (Mycothiol S-conjugate amidase Mca) is predicted to be co-regulated in modules bicluster_0306 with residual 0.50 and bicluster_0335 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 2.90 for bicluster_0306 and 57.00 and 1,600.00 for bicluster_0335 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14885 MT1113 166
Product (LegacyBRC) Product (RefSeq)
Mycothiol conjugate amidase Mca [Mycothiol S-conjugate amidase] Mycothiol conjugate amidase Mca (Mycothiol S-conjugate amidase)
Operon # Operon
729 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608222 NP_215598.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008270

zinc ion binding

zinc ion binding

Details: 
Interacting selectively and non-covalently with zinc (Zn) ions.
GO Category: 
molecular_function
15
Total items in this category:  
GO:0010126

mycothiol metabolic process

mycothiol metabolic process

Details: 
The chemical reactions and pathways involving mycothiol, which consists of N-acetyl-L-cysteine linked to a pseudodisaccharide, D-glucosamine and myo-inositol. Mycothiol is produced in actinomycetes like mycobacteria and serves similar functions to glutathione.
GO Category: 
biological_process
1
Total items in this category:  
GO:0010127

mycothiol-dependent detoxification

mycothiol-dependent detoxification

Details: 
The chemical reactions using mycothiol to convert an alkylating agent to an S-conjugate of mycothiol. The latter is cleaved to release mercapturic acid which is excreted from the cell.
GO Category: 
biological_process
2
Total items in this category:  
GO:0016810

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Details: 
Catalysis of the hydrolysis of any carbon-nitrogen bond, C-N, with the exception of peptide bonds.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0016811

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Details: 
Catalysis of the hydrolysis of any non-peptide carbon-nitrogen bond in a linear amide.
GO Category: 
molecular_function
5
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.070000 0.79

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: