Rv1094 Fatty acid desaturase occurring in virulence cluster

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1094 desA2 Fatty acid desaturase occurring in virulence cluster CDS 1221959 1222786 + 828 275 FALSE

Rv1094 (Fatty acid desaturase occurring in virulence cluster) is predicted to be co-regulated in modules bicluster_0276 with residual 0.47 and bicluster_0426 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 93.00 and 170.00 for bicluster_0276 and 950.00 and 11,000.00 for bicluster_0426 respectively.

These modules are enriched for following go terms: drug transmembrane transporter activity, drug transporter activity nicotinamide nucleotide metabolic proces..., oxidoreduction coenzyme metabolic proces..., pyridine nucleotide metabolic process, pyridine-containing compound metabolic p..., RNA binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE ACYL-[ACYL-CARRIER PROTEIN] DESATURASE DESA2 [ACYL-[ACP] DESATURASE] [STEAROYL-ACP DESATURASE] acyl-[acyl-carrier protein] desaturase
Operon # Operon
739
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Biosynthesis of unsaturated fatty acids

10
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608234 NP_215610.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0046872

metal ion binding

metal ion binding

Details: 
Interacting selectively and non-covalently with any metal ion.
GO Category: 
molecular_function
5
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: