Rv1143 Alpha-methylacyl-CoA racemase (EC 5.1.99.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1143 mcr Alpha-methylacyl-CoA racemase (EC 5.1.99.4) CDS 1270062 1271144 + 1 083 360 FALSE

Rv1143 (Alpha-methylacyl-CoA racemase (EC 5.1.99.4)) is predicted to be co-regulated in modules bicluster_0218 with residual 0.55 and bicluster_0499 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0218 and 560.00 and 11,000.00 for bicluster_0499 respectively.

These modules are enriched for following go terms: aspartic-type endopeptidase activity, aspartic-type peptidase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18417 MT1176 2232
Product (LegacyBRC) Product (RefSeq)
PROBABLE ALPHA-METHYLACYL-CoA RACEMASE MCR [2-methylacyl-CoA racemase] [2-arylpropionyl-CoA epimerase ] alpha-methylacyl-CoA racemase
Operon # Operon
774 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Alpha-methylacyl-CoA racemase Primary bile acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608283 NP_215659.1 Run
GO:0008111

alpha-methylacyl-CoA racemase activity

alpha-methylacyl-CoA racemase activity

Details: 
Catalysis of the reaction: (2S)-2-methylacyl-CoA = (2R)-2-methylacyl-CoA.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.600000 1.57

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: