Rv1144 3-hydroxyacyl-CoA dehydrogenase [isoleucine degradation] (EC

Product Feature Type Start End Strand Length AA Length is TF
Rv1144 3-hydroxyacyl-CoA dehydrogenase [isoleucine degradation] (EC CDS 1271156 1271908 + 753 250 FALSE

Rv1144 (3-hydroxyacyl-CoA dehydrogenase [isoleucine degradation] (EC is predicted to be co-regulated in modules bicluster_0218 with residual 0.55 and bicluster_0499 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0218 and 560.00 and 11,000.00 for bicluster_0499 respectively.

These modules are enriched for following go terms: aspartic-type endopeptidase activity, aspartic-type peptidase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18891 MT1177 3260
Product (LegacyBRC) Product (RefSeq)
PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE_REDUCTASE short-chain type dehydrogenase/reductase
Operon # Operon
774 -
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15608284 NP_215660.1 Run

plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.470000 1.32

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: