Rv1305 ATP synthase C chain (EC 3.6.3.14)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1305 atpE ATP synthase C chain (EC 3.6.3.14) CDS 1461045 1461290 + 246 81 FALSE

Rv1305 (ATP synthase C chain (EC 3.6.3.14)) is predicted to be co-regulated in modules bicluster_0030 with residual 0.60 and bicluster_0586 with residual 0.63.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.05 and 0.81 for bicluster_0030 and 1,800.00 and 5,500.00 for bicluster_0586 respectively.

These modules are enriched for following go terms: single-organism metabolic process, single-organism cellular process, biosynthetic process, cellular nitrogen compound metabolic pro..., nitrogen compound metabolic process, membrane part nucleic acid metabolic process, heterocyclic compound binding, organic cyclic compound binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
ATP synthase subunit c F0F1 ATP synthase subunit C
Operon # Operon
887 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608445 NP_215821.1 Run
GO:0046933

hydrogen ion transporting ATP synthase activity, rotational mechanism

hydrogen ion transporting ATP synthase activity, rotational mechanism

Details: 
Catalysis of the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ADP + phosphate = ATP + H2O, coupled with transport of H+ down a concentration gradient, by a rotational mechanism.
GO Category: 
molecular_function
8
Total items in this category:  
GO:0046961

proton-transporting ATPase activity, rotational mechanism

proton-transporting ATPase activity, rotational mechanism

Details: 
Catalysis of the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out), by a rotational mechanism.
GO Category: 
molecular_function
8
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: