Rv1483 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1483 fabG1 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) CDS 1673440 1674183 + 744 247 FALSE

Rv1483 (3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)) is predicted to be co-regulated in modules bicluster_0012 with residual 0.58 and bicluster_0477 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 0.11 for bicluster_0012 and 0.08 and 0.29 for bicluster_0477 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0012
e.value: 
3.3
Motif Bicluster: 
e.value: 
0.11
Motif Bicluster: 
0.58
bicluster_0477
e.value: 
0.082
Motif Bicluster: 
e.value: 
0.29
Motif Bicluster: 
0.44
Product (LegacyBRC) Product (RefSeq)
3-oxoacyl-[acyl-carrier-protein] reductase 3-oxoacyl-[acyl-carrier protein] reductase FabG1
Operon # Operon
987 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

3-oxoacyl-[acyl-carrier-protein] reductase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608621 NP_215999.1 Run
GO:0004316

3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

Details: 
Catalysis of the reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004316

3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity

Details: 
Catalysis of the reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0018454

acetoacetyl-CoA reductase activity

acetoacetyl-CoA reductase activity

Details: 
Catalysis of the reaction: (R)-3-hydroxyacyl-CoA + NADP+ = 3-oxoacyl-CoA + NADPH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0051289

protein homotetramerization

protein homotetramerization

Details: 
The formation of a protein homotetramer, a macromolecular structure consisting of four noncovalently associated identical subunits.
GO Category: 
biological_process
17
Total items in this category:  
GO:0071768

mycolic acid biosynthetic process

mycolic acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of mycolic acids, beta-hydroxy fatty acids with a long alpha-alkyl side chain.
GO Category: 
biological_process
15
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: